Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay

greiner-lab

Evaluation of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay: A Comparative Research of 4 Isolation Kits

There’s a lack of dependable biomarkers for issues of the central nervous system (CNS), and diagnostics nonetheless closely depend on signs which can be each subjective and tough to quantify. The cerebrospinal fluid (CSF) is a promising supply of biomarkers as a result of its shut connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic evaluation of CSF extracellular vesicles (EVs) and their molecular composition probably displays modifications within the CNS to the next extent in contrast with complete CSF, particularly within the case of neuroinflammation, which may improve blood-brain barrier permeability and trigger an inflow of plasma proteins into the CSF. We used proximity extension assay for proteomic evaluation as a result of its excessive sensitivity. We imagine that this technique might be helpful for de novo biomarker discovery for a number of CNS illnesses. We in contrast 4 commercially out there kits for EV isolation: MagCapture and ExoIntact (primarily based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The remoted EVs had been characterised by nanoparticle monitoring evaluation, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) utilizing two completely different panels, every consisting of 92 markers. The exoEasy samples didn’t go the built-in quality control and had been excluded from downstream evaluation. The variety of detectable proteins within the ExoIntact samples was significantly greater (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) in contrast with different teams. ExoIntact additionally confirmed the very best intersample correlation with a mean Pearson’s correlation coefficient of 0.991 in contrast with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Evaluating complete CSF and ExoIntact samples revealed 70 differentially expressed proteins within the cardiovascular III panel and 17 within the cell regulation panel. To our information, that is the primary time that CSF EVs had been analyzed by PEA. In conclusion, evaluation of CSF EVs by PEA is possible, and completely different isolation kits give distinct outcomes, with ExoIntact exhibiting the very best variety of recognized proteins with the bottom variability.

Efficiency of the QPLEX™ Alz plus assay, a novel multiplex package for screening cerebral amyloid deposition

Background: Alzheimer’s illness (AD) is an irreversible neurodegenerative illness characterised by the hallmark discovering of cerebral amyloid deposition. Many researchers have tried to foretell the existence of cerebral amyloid deposition by utilizing simply accessible blood plasma samples, however the effectiveness of such methods stays controversial. Strategies: We developed a brand new multiplex package, the QPLEX™ Alz plus assay package, which makes use of proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A complete of 300 individuals who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which permits imaging of cerebral amyloid deposition had been included on this examine. We in contrast the degrees of QPLEX™ biomarkers between sufferers who had been categorized as PiB-negative or PiB-positive, no matter their cognitive perform. Logistic regression evaluation adopted by receiver working attribute (ROC) curve evaluation was carried out. The package accuracy was examined utilizing a randomized pattern choice methodology. Outcomes: The outcomes obtained utilizing our assay package reached 89.1% space underneath curve (AUC) with 80.0% sensitivity and 83.0% specificity. Additional validation of the QPLEX™ Alz plus assay package utilizing a randomized pattern choice methodology confirmed a mean accuracy of 81.5%. Conclusions: Our QPLEX™ Alz plus assay package offers preliminary proof that it may be used as blood marker to foretell cerebral amyloid deposition however impartial validation is required.

greiner-lab
greiner-lab

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests 25 tests
EUR 698.4

SR-101-Leu-CMK FLISP™ Assay Kit

KF17321 100 Tests
EUR 834

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests 25 tests
EUR 679.2

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN 1/pk
EUR 1262.4
Description: Lab Equipment; Axygen Branded EQ

SR-101-Phe-CMK FLISP™ Assay Kit

KF17318 25 Tests
EUR 349.2

SR-101-Phe-CMK FLISP™ Assay Kit

KF17319 100 Tests
EUR 834

Polycaspase Assay Kit, green

KF17200 25 Tests
EUR 364.8

Malachite Green Phosphate Assay Kit

abx298878-100Assays 100 Assays
EUR 453.6

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

Malachite Green Phosphate Assay Kit

POMG-25H 2500
EUR 330
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Malachite Green Phosphate Assay Kit

Z5030012 2,500 assays
EUR 410.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Malachite Green Phosphate Assay kit

65-POMG-25H 2500 tests
EUR 333

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests 25 tests
EUR 679.2

FAM-cAMP PDE IV substrate *Green Fluorescence*

13602 0.5 umol
EUR 367.2

FAM-cGMP PDE V substrate *Green Fluorescence*

13604 0.5 umol
EUR 367.2

Caspase 3 & 7 Assay Kit, green

KF17203 25 Tests
EUR 364.8

EZCell? Phagocytosis Assay Kit (Green Zymosan)

K397-100
EUR 516

Morpheus HT-96 Green Screen

M-MD1-47-GREEN 96 x 1 ml ml
EUR 280
Description: Morpheus HT-96 Green Screen

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

EZCell? Phagocytosis Assay Kit (Green E. coli)

K963-100
EUR 516

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342 96 assays
EUR 762
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342-5 5 x 96 assays
EUR 3058.8
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

STA-349 192 assays
EUR 644.4
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0001 1.0mg
EUR 385.2
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0005 5.0mg
EUR 1430.4
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Dap/ Rat Dap ELISA Kit

ELI-31052r 96 Tests
EUR 1063.2

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 200 Tests
EUR 262.8

Amplite™ Fluorimetric Glutathione Assay Kit *Green Fluorescence*

10055 200 Tests
EUR 262.8

Cell Meter™ Autophagy Assay Kit *Green Fluorescence*

23002 200 Tests
EUR 367.2

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0001 1.0mg
EUR 441.6
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0005 5.0mg
EUR 1662
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0001 1.0mg
EUR 400.8
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0005 5.0mg
EUR 1488
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0001 1.0mg
EUR 283.2
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0005 5.0mg
EUR 1023.6
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Canadian Pesticide Kit Containing All 6 Mixes

CAN-CAN-KIT 1ML
EUR 758.1

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0001 1.0mg
EUR 445.2
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0005 5.0mg
EUR 1585.2
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*

13512 100 Tests
EUR 367.2

Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

13601 200 Tests
EUR 262.8

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
EUR 262.8

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 500 Tests
EUR 367.2

Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*

22786 500 Tests
EUR 262.8

Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*

22849 50 Tests
EUR 367.2

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100
EUR 588

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100
EUR 588

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100
EUR 588

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 414
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347 96 assays
EUR 734.4
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347-5 5 x 96 assays
EUR 2913.6
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

5-FAM

20-abx095073
  • EUR 627.60
  • EUR 444.00
  • EUR 1528.80
  • 1 g
  • 500 mg
  • 5 g

6-FAM

HY-66021 500mg
EUR 374.4

5-FAM

HY-66022 10mM/1mL
EUR 151.2

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0001 1.0mg
EUR 529.2
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0005 5.0mg
EUR 2010
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5524 200 Tests
EUR 262.8

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

13456 100 tests
EUR 367.2

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

13500 500 Tests
EUR 262.8

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*

13503 500 Tests
EUR 367.2

Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

13510 100 Tests
EUR 367.2

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*

16315 100 Tests
EUR 420

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

22730 200 Tests
EUR 262.8

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100
EUR 660

Cell Meter™ Cellular Senescence Activity Assay Kit *Green Fluorescence*

23005 100 Tests
EUR 334

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0001 1.0mg
EUR 385.2
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0005 5.0mg
EUR 1430.4
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

DAP Antibody

43505-100ul 100ul
EUR 302.4

DAP antibody

70R-16735 50 ul
EUR 522
Description: Rabbit polyclonal DAP antibody

DAP antibody

70R-32429 100 ug
EUR 392.4
Description: Rabbit polyclonal DAP antibody

DAP antibody

38736-100ul 100ul
EUR 302.4

DAP Antibody

35710-100ul 100ul
EUR 302.4

DAP Antibody

ABD7628 100 ug
EUR 525.6

DAP siRNA

20-abx901410
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx913570
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx913571
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

DAP Antibody

7389-002mg 0.02 mg
EUR 206.18
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

DAP Antibody

7389-01mg 0.1 mg
EUR 523.7
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

DAP Peptide

7389P 0.05 mg
EUR 197.7
Description: (CT) DAP Peptide

DAP Antibody

DF7628 200ul
EUR 420

DAP Antibody

1-CSB-PA170450
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

DAP Antibody

1-CSB-PA262326
  • EUR 380.40
  • EUR 292.80
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DAP Antibody

1-CSB-PA006496GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA

DAP Antibody

1-CSB-PA007640
  • EUR 266.40
  • EUR 234.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

DAP Antibody

1-CSB-PA01034A0Rb
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:50-1:200

Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5528 200 Tests
EUR 262.8

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*

11503 200 Tests
EUR 315.6

Amplite™ Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*

10056 200 Tests
EUR 367.2

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

16060 200 Tests
EUR 367.2

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*

22798 200 Tests
EUR 262.8

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

22799 200 Tests
EUR 367.2

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K714-100
EUR 614.4

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K560-100
EUR 588

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K592-100
EUR 588

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T 20 assays
EUR 400.8
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

FAM-LETD-FMK

13416 100 ug
EUR 315.6

FAM-VAD-FMK

13470 25 Tests
EUR 158.4

6 FAM SE

A8771-10 10 mg
EUR 208.8
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-100 100 mg
EUR 877.2
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-5.1 10 mM (in 1mL DMSO)
EUR 238.8
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-50 50 mg
EUR 543.6
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

5-FAM Azide

950 100 mg
EUR 842.4

5-FAM Alkyne

951 100 mg
EUR 842.4

6-FAM Azide

955 100 mg
EUR 842.4

6-FAM Alkyne

956 100 mg
EUR 842.4

3?-FAM CPG

AHP-CH-FAM0103 100 mg Ask for price

3?-FAM CPG

AHP-CH-FAM1003 1000 mg Ask for price

5-FAM SE

HY-15938 100mg
EUR 1448.4

6-FAM SE

HY-15939 100mg
EUR 734.4

5-FAM-Woodtide

H-7644.0001 1.0mg
EUR 472.8
Description: Sum Formula: C89H133N21O26S; CAS# [1566528-51-5] net

FAM-VAD-FMK

13484 100 Tests
EUR 211

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

K2085-100 100 assays
EUR 634.8

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

K564-100
EUR 646.8

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Islr ELISA Kit| Mouse Immunoglobulin superfamily containing leu

EF015231 96 Tests
EUR 826.8

Sperm chromatin construction assay versus sperm chromatin dispersion kits: Technical repeatability and selection of assisted reproductive know-how process

Goal: The sperm DNA fragmentation index (DFI) guides the clinician’s selection of an acceptable assisted reproductive know-how (ART) process. The DFI may be decided utilizing commercially out there methodologies, together with sperm chromatin dispersion (SCD) kits and sperm chromatin construction assay (SCSA). At the moment, when DFI is evaluated utilizing SCD kits, the result’s analyzed in reference to the SCSA-derived threshold for the selection of an ART process. On this examine, we in contrast DFI values obtained utilizing SCSA with these obtained utilizing SCD and decided whether or not the distinction impacts the selection of ART process. Strategies: We in contrast SCSA to 2 SCD kits, CANfrag (n=36) and Halosperm (n=31), to evaluate the DFI values obtained, the correlations between assessments, the technical repeatability, and the affect of DFI on the selection of ART. Outcomes: We obtained greater median DFI values utilizing SCD kits than when utilizing SCSA, and this distinction was vital for the CANfrag package (p<0.001). The SCD kits had considerably greater coefficients of variation than SCSA (p<0.0001). In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) could be chosen for a considerably greater proportion of sufferers if a call had been made primarily based on DFI derived from SCD quite than DFI decided utilizing SCSA (p=0.003). Conclusion: Our outcomes point out that SCD kit-specific thresholds ought to be established so as to keep away from the pointless use of IVF/ICSI primarily based on sperm DNA harm for the administration of infertility. Acceptable measures ought to be taken to mitigate the elevated variability inherent to the strategies utilized in these assessments.

A easy and speedy algal assay package to evaluate toxicity of heavy metal-contaminated water

This examine presents a novel algal-based toxicity take a look at appropriate for easy and speedy evaluation of heavy metallic (Hg2+, Cr6+, Cd2+, Pb2+, or As3+)-contaminated water. A closed-system kit-type algal assay was developed utilizing Chlorella vulgaris. Toxicity was assessed by oxygen evolution within the gaseous section of the assay kits, which was measured through a needle-type oxygen sensor. Preliminary cell density, gentle depth, and publicity time that enabled favorable take a look at efficiency for the algal assay kits had been 103 cells/mL, 250 μmol m-2s-1, and 18 h, respectively. Outcomes from the heavy metallic toxicity assessments reveal that Hg2+, Cr6+, Cd2+, and Pb2+ are extra poisonous in inhibiting algal photosynthetic exercise than As3+. The 18 h half-maximum efficient concentrations (EC50) for Hg2+, Cr6+, Cd2+, Pb2+, and As3+ had been decided to be 31.3 ± 0.5, 179.6 ± 7.5, 301.3 ± 6.1, 476.1 ± 10.5, and 2184.1 ± 31.1 μg/L, respectively. A robust correlation between oxygen concentrations within the headspace of the assay kits and chlorophyll a manufacturing signifies that oxygen evolution within the gaseous section is capable of symbolize algal photosynthetic exercise and function the end-point in algal toxicity assessments. Excessive take a look at sensitivity and reproducibility in addition to a straightforward take a look at protocol and speedy processing time make the algal assay package an appropriate instrument for easy and speedy toxicity testing of heavy metal-contaminated water.    

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 616.8

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 283.2

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 408

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 390

Caspase-1 Fluorometric Assay Kit

K110-100
EUR 574.8

Caspase-1 Fluorometric Assay Kit

K110-200
EUR 744

Caspase-1 Fluorometric Assay Kit

K110-25
EUR 248.4

Caspase-1 Fluorometric Assay Kit

K110-400
EUR 1149.6

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 574.8

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 744

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 248.4

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 1149.6

Caspase-1 Fluorometric Assay Kit

K2010-100 100 assays
EUR 568.8

Caspase-1 Fluorometric Assay Kit

K2010-200 200 assays
EUR 835.2

Caspase-1 Fluorometric Assay Kit

K2010-25 25 assays
EUR 285.6

Caspase-1 Fluorometric Assay Kit

K2010-400 400 assays
EUR 1303.2

Caspase-1 Colorimetric Assay Kit

K2011-100 100 assays
EUR 568.8

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 835.2

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 285.6

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1303.2

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-1 1KIT
EUR 120
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-1 1KIT
EUR 120
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 393.6
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Caspase 3 Assay Kit

20-abx294003
  • EUR 1506.00
  • EUR 548.40
  • EUR 924.00
  • 100 tests
  • 20 tests
  • 50 tests

Poly-Caspase Assay Kit

20-abx299016
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 6 Assay Kit

20-abx299018
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Poly-Caspase Assay Kit

abx299022-2550tests 25-50 tests
EUR 830.4

Poly-Caspase Assay Kit

20-abx299025
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 698.4

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1562.4

Caspase 9 Assay Kit

20-abx299035
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Glycerol Cell-Based Assay Kit

K977-100
EUR 588

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 244.8
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 1 Colorimetric Cell-Based ELISA Kit

EKC1083 100ul
EUR 686.4

Caspase 3 Fluorometric Assay Kit

55R-1269 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit

55R-1274 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 344.4
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit

55R-1278 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 332.4
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 355.2
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit

55R-1282 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit

55R-1284 25 assays
EUR 340.8
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit

55R-1286 25 assays
EUR 363.6
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 374.4
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 3/7 Assay Kit

20-abx299017
  • EUR 1562.40
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 830.4

Caspase 3/7 Assay Kit

20-abx299028
  • EUR 1431.60
  • EUR 698.40
  • 100 tests
  • 25 tests

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 616.8

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 283.2

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 408

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 330
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 602.4

Caspase-5 Fluorometric Assay Kit

K2195-200 200 assays
EUR 835.2

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 285.6

Caspase-5 Fluorometric Assay Kit

K2195-400 400 assays
EUR 1336.8

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 634.8

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 870

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 301.2

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1387.2

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 634.8

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 885.6

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 301.2

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1336.8

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 634.8

Caspase-4 Fluorometric Assay Kit

K2198-200 200 assays
EUR 885.6

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 301.2

Caspase-4 Fluorometric Assay Kit

K2198-400 400 assays
EUR 1336.8

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 668.4

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 885.6

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 319.2

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1336.8

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 364.8

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 364.8

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 364.8

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 364.8

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 364.8

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 364.8

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 364.8

Caspase-12 Fluorometric Assay Kit

K139-100
EUR 601.2

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 307.2

Caspase-4 Fluorometric Assay Kit

K126-100
EUR 561.6

Caspase-4 Fluorometric Assay Kit

K126-200
EUR 738

Caspase-4 Fluorometric Assay Kit

K126-25
EUR 255.6

Caspase-4 Fluorometric Assay Kit

K126-400
EUR 1110

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 561.6

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 738

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 274.8

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 1149.6

Caspase-5 Fluorometric Assay Kit

K122-100
EUR 523.2

Caspase-5 Fluorometric Assay Kit

K122-200
EUR 698.4

Caspase-5 Fluorometric Assay Kit

K122-25
EUR 242.4

Caspase-5 Fluorometric Assay Kit

K122-400
EUR 1149.6

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 561.6

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 738

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 255.6

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 1149.6

Caspase-10 Fluorometric Assay Kit

K124-100
EUR 529.2

Caspase-10 Fluorometric Assay Kit

K124-200
EUR 738

Caspase-10 Fluorometric Assay Kit

K124-25
EUR 242.4

Caspase-10 Fluorometric Assay Kit

K124-400
EUR 1149.6

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 555.6

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 738

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 255.6

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 1149.6

Caspase-8 Fluorometric Assay Kit

K112-100
EUR 574.8

Caspase-8 Fluorometric Assay Kit

K112-200
EUR 738

Caspase-8 Fluorometric Assay Kit

K112-25
EUR 248.4

Caspase-8 Fluorometric Assay Kit

K112-400
EUR 1149.6

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 574.8

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 744

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 248.4

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 1149.6

Caspase-6 Fluorometric Assay Kit

K114-100
EUR 516

Caspase-6 Fluorometric Assay Kit

K114-200
EUR 738

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 242.4

Caspase-6 Fluorometric Assay Kit

K114-400
EUR 1149.6

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 542.4

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 738

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 242.4

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 1149.6

Caspase-2 Fluorometric Assay Kit

K116-100
EUR 555.6

Caspase-2 Fluorometric Assay Kit

K116-200
EUR 738

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 242.4

Caspase-2 Fluorometric Assay Kit

K116-400
EUR 1149.6

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 561.6

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 738

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 255.6

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 1149.6

Caspase-3 Fluorometric Assay Kit

K105-100
EUR 574.8

Caspase-3 Fluorometric Assay Kit

K105-200
EUR 744

Caspase-3 Fluorometric Assay Kit

K105-25
EUR 248.4

Caspase-3 Fluorometric Assay Kit

K105-400
EUR 1174.8

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 574.8

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 757.2

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 248.4

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 1149.6

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 561.6

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 738

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 248.4

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 1149.6

Caspase-9 Colorimetric Assay Kit

K119-100
EUR 588

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 744

Caspase-9 Colorimetric Assay Kit

K119-25
EUR 261.6

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 1149.6

Caspase-3 Fluorometric Assay Kit

K2007-100 100 assays
EUR 568.8

Caspase-3 Fluorometric Assay Kit

K2007-200 200 assays
EUR 835.2

Caspase-3 Fluorometric Assay Kit

K2007-25 25 assays
EUR 285.6

Caspase-3 Fluorometric Assay Kit

K2007-400 400 assays
EUR 1303.2

Caspase-3 Colorimetric Assay Kit

K2008-100 100 assays
EUR 568.8

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 835.2

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 285.6

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1303.2

Caspase-8 Fluorometric Assay Kit

K2012-100 100 assays
EUR 568.8

Caspase-8 Fluorometric Assay Kit

K2012-200 200 assays
EUR 835.2

Caspase-8 Fluorometric Assay Kit

K2012-25 25 assays
EUR 285.6

Caspase-8 Fluorometric Assay Kit

K2012-400 400 assays
EUR 1303.2

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 568.8

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 835.2

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 285.6

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1303.2

Caspase-6 Fluorometric Assay Kit

K2014-100 100 assays
EUR 568.8

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 835.2

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 285.6

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 1303.2

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 584.4

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 835.2

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 285.6

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1321.2

Caspase-2 Fluorometric Assay Kit

K2016-100 100 assays
EUR 602.4

Caspase-2 Fluorometric Assay Kit

K2016-200 200 assays
EUR 835.2

Caspase-2 Fluorometric Assay Kit

K2016-25 25 assays
EUR 285.6

Caspase-2 Fluorometric Assay Kit

K2016-400 400 assays
EUR 1336.8

Caspase-2 Colorimetric Assay Kit

K2017-100 100 assays
EUR 634.8

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 870

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 301.2

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1387.2

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 602.4

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 802.8

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 285.6

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1336.8

Caspase-9 Colorimetric Assay Kit

K2019-100 100 assays
EUR 634.8

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