Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay

greiner-lab

Evaluation of Cerebrospinal Fluid Extracellular Vesicles by Proximity Extension Assay: A Comparative Research of 4 Isolation Kits

There’s a lack of dependable biomarkers for issues of the central nervous system (CNS), and diagnostics nonetheless closely depend on signs which can be each subjective and tough to quantify. The cerebrospinal fluid (CSF) is a promising supply of biomarkers as a result of its shut connection to the CNS. Extracellular vesicles are actively secreted by cells, and proteomic evaluation of CSF extracellular vesicles (EVs) and their molecular composition probably displays modifications within the CNS to the next extent in contrast with complete CSF, particularly within the case of neuroinflammation, which may improve blood-brain barrier permeability and trigger an inflow of plasma proteins into the CSF. We used proximity extension assay for proteomic evaluation as a result of its excessive sensitivity. We imagine that this technique might be helpful for de novo biomarker discovery for a number of CNS illnesses. We in contrast 4 commercially out there kits for EV isolation: MagCapture and ExoIntact (primarily based on magnetic beads), EVSecond L70 (size-exclusion chromatography), and exoEasy (membrane affinity). The remoted EVs had been characterised by nanoparticle monitoring evaluation, ELISA (CD63, CD81 and albumin), and proximity extension assay (PEA) utilizing two completely different panels, every consisting of 92 markers. The exoEasy samples didn’t go the built-in quality control and had been excluded from downstream evaluation. The variety of detectable proteins within the ExoIntact samples was significantly greater (~150% for the cardiovascular III panel and ~320% for the cell regulation panel) in contrast with different teams. ExoIntact additionally confirmed the very best intersample correlation with a mean Pearson’s correlation coefficient of 0.991 in contrast with 0.985 and 0.927 for MagCapture and EVSecond, respectively. The median coefficient of variation was 5%, 8%, and 22% for ExoIntact, MagCapture, and EVSecond, respectively. Evaluating complete CSF and ExoIntact samples revealed 70 differentially expressed proteins within the cardiovascular III panel and 17 within the cell regulation panel. To our information, that is the primary time that CSF EVs had been analyzed by PEA. In conclusion, evaluation of CSF EVs by PEA is possible, and completely different isolation kits give distinct outcomes, with ExoIntact exhibiting the very best variety of recognized proteins with the bottom variability.

Efficiency of the QPLEX™ Alz plus assay, a novel multiplex package for screening cerebral amyloid deposition

Background: Alzheimer’s illness (AD) is an irreversible neurodegenerative illness characterised by the hallmark discovering of cerebral amyloid deposition. Many researchers have tried to foretell the existence of cerebral amyloid deposition by utilizing simply accessible blood plasma samples, however the effectiveness of such methods stays controversial. Strategies: We developed a brand new multiplex package, the QPLEX™ Alz plus assay package, which makes use of proteomics-based blood biomarkers to prescreen for cerebral amyloid deposition. A complete of 300 individuals who underwent Pittsburgh compound B (PiB)-positron emission tomography (PET) which permits imaging of cerebral amyloid deposition had been included on this examine. We in contrast the degrees of QPLEX™ biomarkers between sufferers who had been categorized as PiB-negative or PiB-positive, no matter their cognitive perform. Logistic regression evaluation adopted by receiver working attribute (ROC) curve evaluation was carried out. The package accuracy was examined utilizing a randomized pattern choice methodology. Outcomes: The outcomes obtained utilizing our assay package reached 89.1% space underneath curve (AUC) with 80.0% sensitivity and 83.0% specificity. Additional validation of the QPLEX™ Alz plus assay package utilizing a randomized pattern choice methodology confirmed a mean accuracy of 81.5%. Conclusions: Our QPLEX™ Alz plus assay package offers preliminary proof that it may be used as blood marker to foretell cerebral amyloid deposition however impartial validation is required.

greiner-lab
greiner-lab

FAM-Phe-DAP Serine Protease Assay Kit

abx299013-25tests 25 tests
EUR 582

SR-101-Leu-CMK FLISP™ Assay Kit

KF17321 100 Tests
EUR 695

FAM-Leu-CMK Serine Protease Assay Kit

abx299011-25tests 25 tests
EUR 566

OPTIONAL GREEN FILTER (572NM)

GDBL-GREEN 1/pk
EUR 1052
Description: Lab Equipment; Axygen Branded EQ

Polycaspase Assay Kit, green

KF17200 25 Tests
EUR 304

SR-101-Phe-CMK FLISP™ Assay Kit

KF17318 25 Tests
EUR 291

SR-101-Phe-CMK FLISP™ Assay Kit

KF17319 100 Tests
EUR 695

Caspase 1 Assay Kit, green

KF17201 25 Tests
EUR 325

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 304

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 304

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 304

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 304

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 304

Malachite Green Phosphate Assay Kit

abx298878-100Assays 100 Assays
EUR 378

Malachite Green Phosphate Assay Kit

Z5030012 2,500 assays
EUR 342
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Malachite Green Phosphate Assay Kit

POMG-25H 2500
EUR 275
Description: High-throµghput phosphate assay using malachite green method at 620nm. Kit size: 2500 tests. Detection limit: 0.02 µM. Shelf life: 12 months. Shipping: ambient temp; storage: 4°C.

Caspase 3 & 7 Assay Kit, green

KF17203 25 Tests
EUR 304

EZCell? Phagocytosis Assay Kit (Green Zymosan)

K397-100
EUR 430

FAM-Phe-CMK Serine Protease Assay Kit

abx299010-25tests 25 tests
EUR 566

FAM-cAMP PDE IV substrate *Green Fluorescence*

13602 0.5 umol
EUR 306

FAM-cGMP PDE V substrate *Green Fluorescence*

13604 0.5 umol
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342 96 assays
EUR 635
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342-5 5 x 96 assays
EUR 2549
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

STA-349 192 assays
EUR 537
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

EZCell? Phagocytosis Assay Kit (Green E. coli)

K963-100
EUR 430

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0001 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-1895.0005 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [140430-53-1] net

Amplite™ Fluorimetric Acetylcholinesterase Assay Kit *Green Fluorescence*

11401 200 Tests
EUR 219

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0001 1.0mg
EUR 368
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2350.0005 5.0mg
EUR 1385
Description: Sum Formula: C55H80N16O16; CAS# [720710-69-0] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0001 1.0mg
EUR 334
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Mca-Pro-Leu-Gly-Leu-Glu-Glu-Ala-Dap(Dnp)-NH2

M-2670.0005 5.0mg
EUR 1240
Description: Sum Formula: C53H70N12O20; CAS# [891198-38-2] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0001 1.0mg
EUR 236
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

H-2638.0005 5.0mg
EUR 853
Description: Sum Formula: C50H77N17O13; CAS# [290362-09-3] net

Dap/ Rat Dap ELISA Kit

ELI-31052r 96 Tests
EUR 886

Amplite™ Fluorimetric Alkaline Phosphatase Assay Kit *Green Fluorescence*

11953 500 Tests
EUR 219

Amplite™ Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

12601 500 Tests
EUR 306

Amplite™ MMP-3 Activity Assay Kit *Green Fluorescence*

13512 100 Tests
EUR 306

Amplite™ Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

13601 200 Tests
EUR 219

Cell Meter™ Cell Viability Assay Kit *Green Fluorescence*

22786 500 Tests
EUR 219

Cell Meter™ TUNEL Apoptosis Assay Kit *Green Fluorescence*

22849 50 Tests
EUR 306

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 345
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347 96 assays
EUR 612
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347-5 5 x 96 assays
EUR 2428
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? Palmitoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K452-100
EUR 490

EZClick? Stearoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K453-100
EUR 490

EZClick? Myristoylated Protein Assay Kit (FACS/Microscopy), Green Fluorescence

K497-100
EUR 490

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0001 1.0mg
EUR 371
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2225.0005 5.0mg
EUR 1321
Description: Sum Formula: C61H89N17O17; CAS# [396717-35-4] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0001 1.0mg
EUR 441
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

N-Me-Abz-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2

M-2145.0005 5.0mg
EUR 1675
Description: Sum Formula: C51H79N17O13; CAS# [396716-07-7] net

Amplite™ Fluorimetric Proteasome 20S Activity Assay Kit *Green Fluorescence*

13456 100 tests
EUR 306

Amplite™ Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

13500 500 Tests
EUR 219

Amplite™ Fluorimetric Caspase 3/7 Assay Kit *Green Fluorescence*

13503 500 Tests
EUR 306

Amplite™ Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

13510 100 Tests
EUR 306

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*

16315 100 Tests
EUR 350

Cell Navigator™ Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*

22730 200 Tests
EUR 219

Amplite™ Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5524 200 Tests
EUR 219

EZClick? Global Protein Synthesis Assay Kit (FACS/Microscopy), Green Fluorescence

K459-100
EUR 550

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0001 1.0mg
EUR 321
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2

M-2520.0005 5.0mg
EUR 1192
Description: Sum Formula: C49H68N14O15; CAS# [256394-92-0] net

Cell Meter™ Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*

11503 200 Tests
EUR 263

Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

16060 200 Tests
EUR 306

Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*

22798 200 Tests
EUR 219

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*

22799 200 Tests
EUR 306

Amplite™ Rapid Fluorimetric Total Thiol Quantitation Assay Kit *Green Fluorescence*

5528 200 Tests
EUR 219

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T 20 assays
EUR 334
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

EZClick? O-GalNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K560-100
EUR 490

EZClickTM Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K592-100
EUR 490

EZClick? O-GlcNAc Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K714-100
EUR 512

6-FAM

HY-66021 500mg
EUR 312

5-FAM

HY-66022 10mM/1mL
EUR 126

5-FAM

20-abx095073
  • EUR 523.00
  • EUR 370.00
  • EUR 1274.00
  • 1 g
  • 500 mg
  • 5 g

DAP Antibody

1-CSB-PA262326
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:50-1:200

DAP Antibody

1-CSB-PA01034A0Rb
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:50-1:200

DAP Antibody

1-CSB-PA006496GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA

DAP Antibody

1-CSB-PA007640
  • EUR 222.00
  • EUR 195.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/40000

DAP Antibody

1-CSB-PA170450
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against DAP. Recognizes DAP from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:25-1:100

DAP Antibody

35710-100ul 100ul
EUR 252

DAP siRNA

20-abx913570
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx913571
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP siRNA

20-abx901410
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

DAP antibody

38736-100ul 100ul
EUR 252

DAP Antibody

43505-100ul 100ul
EUR 252

DAP Antibody

ABD7628 100 ug
EUR 438

DAP Antibody

DF7628 200ul
EUR 304
Description: DAP Antibody detects endogenous levels of total DAP.

DAP Peptide

7389P 0.05 mg
EUR 164.75
Description: (CT) DAP Peptide

DAP antibody

70R-16735 50 ul
EUR 435
Description: Rabbit polyclonal DAP antibody

DAP antibody

70R-32429 100 ug
EUR 327
Description: Rabbit polyclonal DAP antibody

DAP Antibody

7389-002mg 0.02 mg
EUR 171.82
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

DAP Antibody

7389-01mg 0.1 mg
EUR 436.42
Description: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1). DAP is also a direct substrate of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2).

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

K2085-100 100 assays
EUR 529

Branched Chain Amino Acid (Leu/Ile/Val) Colorimetric Assay Kit

K564-100
EUR 539

Cell Meter™ Live Cell Caspase 1 Binding Assay Kit *Green Fluorescence*

20108 25 Tests
EUR 306

Cell Meter™ Live Cell Caspase 2 Binding Assay Kit *Green Fluorescence*

20111 25 Tests
EUR 306

Cell Meter™ Live Cell Caspase 8 Binding Assay Kit *Green Fluorescence*

20115 25 Tests
EUR 306

Cell Meter™ Live Cell Caspase 9 Binding Assay Kit *Green Fluorescence*

20117 25 Tests
EUR 306

Cell Meter™ Live Cell Caspase 13 Binding Assay Kit *Green Fluorescence*

20125 25 Tests
EUR 306

Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence*

22789 200 Tests
EUR 219

Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Green Fluorescence*

22796 200 Tests
EUR 219

Cell Meter™ Fluorimetric Intracellular Total ROS Activity Assay Kit*Green Fluorescence*

22900 200 Tests
EUR 219

EZClick? Sialic Acid (ManAz) Modified Glycoprotein Assay Kit (FACS/Microscopy, Green Fluorescence)

K441-100
EUR 490

Islr ELISA Kit| Mouse Immunoglobulin superfamily containing leu

EF015231 96 Tests
EUR 689

FAM-LETD-FMK

13416 100 ug
EUR 263

5-FAM Azide

950 100 mg
EUR 702

5-FAM Alkyne

951 100 mg
EUR 702

6-FAM Azide

955 100 mg
EUR 702

6-FAM Alkyne

956 100 mg
EUR 702

5-FAM SE

HY-15938 100mg
EUR 1207

6-FAM SE

HY-15939 100mg
EUR 612

5-FAM-Woodtide

H-7644.0001 1.0mg
EUR 394
Description: Sum Formula: C89H133N21O26S; CAS# [1566528-51-5] net

6 FAM SE

A8771-10 10 mg
EUR 174
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-100 100 mg
EUR 731
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-5.1 10 mM (in 1mL DMSO)
EUR 199
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

6 FAM SE

A8771-50 50 mg
EUR 453
Description: 6-FAM SE, also known as 6-Carboxyfluorescein N-hydroxysuccinimide ester or 6-Carboxyfluorescein N-succinimidyl ester, is an isomer of carboxyfluorescein and amine-reactive succinimidyl ester carboxyfluorescein.

3?-FAM CPG

AHP-CH-FAM0103 100 mg Ask for price

3?-FAM CPG

AHP-CH-FAM1003 1000 mg Ask for price

Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2

M-2510.0001 1.0mg
EUR 708
Description: Sum Formula: C66H82N16O17S; CAS# [1926163-82-7] net

Mca-Pro-Leu-Ala-Cys(Mob)-Trp-Ala-Arg-Dap(Dnp)-NH2

M-2510.0005 5.0mg
EUR 2739
Description: Sum Formula: C66H82N16O17S; CAS# [1926163-82-7] net

Sperm chromatin construction assay versus sperm chromatin dispersion kits: Technical repeatability and selection of assisted reproductive know-how process

Goal: The sperm DNA fragmentation index (DFI) guides the clinician’s selection of an acceptable assisted reproductive know-how (ART) process. The DFI may be decided utilizing commercially out there methodologies, together with sperm chromatin dispersion (SCD) kits and sperm chromatin construction assay (SCSA). At the moment, when DFI is evaluated utilizing SCD kits, the result’s analyzed in reference to the SCSA-derived threshold for the selection of an ART process. On this examine, we in contrast DFI values obtained utilizing SCSA with these obtained utilizing SCD and decided whether or not the distinction impacts the selection of ART process. Strategies: We in contrast SCSA to 2 SCD kits, CANfrag (n=36) and Halosperm (n=31), to evaluate the DFI values obtained, the correlations between assessments, the technical repeatability, and the affect of DFI on the selection of ART. Outcomes: We obtained greater median DFI values utilizing SCD kits than when utilizing SCSA, and this distinction was vital for the CANfrag package (p<0.001). The SCD kits had considerably greater coefficients of variation than SCSA (p<0.0001). In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) could be chosen for a considerably greater proportion of sufferers if a call had been made primarily based on DFI derived from SCD quite than DFI decided utilizing SCSA (p=0.003). Conclusion: Our outcomes point out that SCD kit-specific thresholds ought to be established so as to keep away from the pointless use of IVF/ICSI primarily based on sperm DNA harm for the administration of infertility. Acceptable measures ought to be taken to mitigate the elevated variability inherent to the strategies utilized in these assessments.

A easy and speedy algal assay package to evaluate toxicity of heavy metal-contaminated water

This examine presents a novel algal-based toxicity take a look at appropriate for easy and speedy evaluation of heavy metallic (Hg2+, Cr6+, Cd2+, Pb2+, or As3+)-contaminated water. A closed-system kit-type algal assay was developed utilizing Chlorella vulgaris. Toxicity was assessed by oxygen evolution within the gaseous section of the assay kits, which was measured through a needle-type oxygen sensor. Preliminary cell density, gentle depth, and publicity time that enabled favorable take a look at efficiency for the algal assay kits had been 103 cells/mL, 250 μmol m-2s-1, and 18 h, respectively. Outcomes from the heavy metallic toxicity assessments reveal that Hg2+, Cr6+, Cd2+, and Pb2+ are extra poisonous in inhibiting algal photosynthetic exercise than As3+. The 18 h half-maximum efficient concentrations (EC50) for Hg2+, Cr6+, Cd2+, Pb2+, and As3+ had been decided to be 31.3 ± 0.5, 179.6 ± 7.5, 301.3 ± 6.1, 476.1 ± 10.5, and 2184.1 ± 31.1 μg/L, respectively. A robust correlation between oxygen concentrations within the headspace of the assay kits and chlorophyll a manufacturing signifies that oxygen evolution within the gaseous section is capable of symbolize algal photosynthetic exercise and function the end-point in algal toxicity assessments. Excessive take a look at sensitivity and reproducibility in addition to a straightforward take a look at protocol and speedy processing time make the algal assay package an appropriate instrument for easy and speedy toxicity testing of heavy metal-contaminated water.    

Caspase-1 Activity Assay Kit

AR4003-100Assays 100 Assays
EUR 514

Caspase-1 Activity Assay Kit

AR4003-25Assays 25 Assays
EUR 236

Caspase-1 Activity Assay Kit

AR4003-50Assays 50 Assays
EUR 340

Caspase-1 Fluorometric Assay Kit

K2010-200 200 assays
EUR 696

Caspase-1 Fluorometric Assay Kit

K2010-25 25 assays
EUR 238

Caspase-1 Fluorometric Assay Kit

K2010-400 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1086

Caspase 1 Fluorometric Assay Kit

55R-1272 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase-1 Fluorometric Assay Kit

K110-100
EUR 479

Caspase-1 Fluorometric Assay Kit

K110-200
EUR 620

Caspase-1 Fluorometric Assay Kit

K110-25
EUR 207

Caspase-1 Fluorometric Assay Kit

K110-400
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 207

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 958

Caspase-3 DEVD-R110 Fluorometric HTS Assay Kit

30009-1 1KIT
EUR 100
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric HTS Assay Kit

30012-1 1KIT
EUR 100
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 3 Assay Kit

20-abx294003
  • EUR 1255.00
  • EUR 457.00
  • EUR 770.00
  • 100 tests
  • 20 tests
  • 50 tests

Poly-Caspase Assay Kit

20-abx299016
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 6 Assay Kit

20-abx299018
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 8 Assay Kit

20-abx299020
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Poly-Caspase Assay Kit

abx299022-2550tests 25-50 tests
EUR 692

Poly-Caspase Assay Kit

20-abx299025
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 2 Assay Kit

abx299026-25tests 25 tests
EUR 582

Caspase 10 Assay Kit

abx299027-100tests 100 tests
EUR 1302

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 12 Assay Kit

55R-1290 25 assays
EUR 328
Description: Assay Kit for detection of Capase 12 activity in the research laboratory

Glycerol Cell-Based Assay Kit

K977-100
EUR 490

Genorise® Caspase-1 Colorimetric Assay Kit

GR107007 100 tests
EUR 380

Genorise® Caspase-1 Fluorometric Assay Kit

GR107008 100 tests
EUR 350

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 1 Colorimetric Cell-Based ELISA Kit

EKC1083 100ul
EUR 572

Caspase 2 Assay Kit, green

KF17202 25 Tests
EUR 304

Caspase 6 Assay Kit, green

KF17204 25 Tests
EUR 304

Caspase 8 Assay Kit, green

KF17205 25 Tests
EUR 304

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 10 Assay Kit, green

KF17207 25 Tests
EUR 304

Caspase 13 Assay Kit, green

KF17208 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

Caspase-2 Activity Assay Kit

AR4004-100Assays 100 Assays
EUR 514

Caspase-2 Activity Assay Kit

AR4004-25Assays 25 Assays
EUR 236

Caspase-2 Activity Assay Kit

AR4004-50Assays 50 Assays
EUR 340

Caspase 3/7 Assay Kit

20-abx299017
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 3/7 Assay Kit

abx299024-2550tests 25-50 tests
EUR 692

Caspase 3/7 Assay Kit

20-abx299028
  • EUR 1193.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase-3 Fluorometric Assay Kit

K2007-200 200 assays
EUR 696

Caspase-3 Fluorometric Assay Kit

K2007-25 25 assays
EUR 238

Caspase-3 Fluorometric Assay Kit

K2007-400 400 assays
EUR 1086

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 238

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1086

Caspase-8 Fluorometric Assay Kit

K2012-200 200 assays
EUR 696

Caspase-8 Fluorometric Assay Kit

K2012-25 25 assays
EUR 238

Caspase-8 Fluorometric Assay Kit

K2012-400 400 assays
EUR 1086

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Fluorometric Assay Kit

K2014-200 200 assays
EUR 696

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Fluorometric Assay Kit

K2016-200 200 assays
EUR 696

Caspase-2 Fluorometric Assay Kit

K2016-25 25 assays
EUR 238

Caspase-2 Fluorometric Assay Kit

K2016-400 400 assays
EUR 1114

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 251

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1156

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1114

Caspase-9 Colorimetric Assay Kit

K2019-200 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-25 25 assays
EUR 251

Caspase-9 Colorimetric Assay Kit

K2019-400 400 assays
EUR 1156

Caspase-12 Fluorometric Assay Kit

K2150-100 100 assays
EUR 529

Caspase-12 Fluorometric Assay Kit

K2150-25 25 assays
EUR 251

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 502

Caspase-5 Fluorometric Assay Kit

K2195-200 200 assays
EUR 696

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 238

Caspase-5 Fluorometric Assay Kit

K2195-400 400 assays
EUR 1114

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 529

Caspase-4 Fluorometric Assay Kit

K2198-200 200 assays
EUR 738

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 251

Caspase-4 Fluorometric Assay Kit

K2198-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

QuantiFluo Caspase-3 Assay Kit

DCS3-100 100
EUR 275
Description: Quantitative determination of the apoptosis target caspase-3 activity by fluorimetry (400/490nm) method. Procedure: 60 min. Kit size: 100 tests. Shelf life: 12 months.

Caspase 3 Fluorometric Assay Kit

55R-1269 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 8 Fluorometric Assay Kit

55R-1274 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Fluorometric Assay Kit

55R-1276 25 assays
EUR 287
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Fluorometric Assay Kit

55R-1278 25 assays
EUR 284
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 5 Fluorometric Assay Kit

55R-1282 25 assays
EUR 284
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Fluorometric Assay Kit

55R-1284 25 assays
EUR 284
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Fluorometric Assay Kit

55R-1286 25 assays
EUR 303
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-3 Fluorometric Assay Kit

K105-100
EUR 479

Caspase-3 Fluorometric Assay Kit

K105-200
EUR 620

Caspase-3 Fluorometric Assay Kit

K105-25
EUR 207

Caspase-3 Fluorometric Assay Kit

K105-400
EUR 979

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 479

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 631

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 207

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 958

Caspase-8 Fluorometric Assay Kit

K112-100
EUR 479

Caspase-8 Fluorometric Assay Kit

K112-200
EUR 615

Caspase-8 Fluorometric Assay Kit

K112-25
EUR 207

Caspase-8 Fluorometric Assay Kit

K112-400
EUR 958

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 479

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 620

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 207

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 958

Caspase-6 Fluorometric Assay Kit

K114-100
EUR 430

Caspase-6 Fluorometric Assay Kit

K114-200
EUR 615

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 202

Caspase-6 Fluorometric Assay Kit

K114-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

Caspase-2 Fluorometric Assay Kit

K116-100
EUR 463

Caspase-2 Fluorometric Assay Kit

K116-200
EUR 615

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 202

Caspase-2 Fluorometric Assay Kit

K116-400
EUR 958

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 468

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 615

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 213

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 958

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 468

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 615

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 958

Caspase-9 Colorimetric Assay Kit

K119-100
EUR 490

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 620

Caspase-9 Colorimetric Assay Kit

K119-25
EUR 218

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 958

Caspase-5 Fluorometric Assay Kit

K122-100
EUR 436

Caspase-5 Fluorometric Assay Kit

K122-200
EUR 582

Caspase-5 Fluorometric Assay Kit

K122-25
EUR 202

Caspase-5 Fluorometric Assay Kit

K122-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 468

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 615

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 213

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 958

Caspase-10 Fluorometric Assay Kit

K124-100
EUR 441

Caspase-10 Fluorometric Assay Kit

K124-200
EUR 615

Caspase-10 Fluorometric Assay Kit

K124-25
EUR 202

Caspase-10 Fluorometric Assay Kit

K124-400
EUR 958

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 463

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 615

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 213

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 958

Caspase-4 Fluorometric Assay Kit

K126-100
EUR 468

Caspase-4 Fluorometric Assay Kit

K126-200
EUR 615

Caspase-4 Fluorometric Assay Kit

K126-25
EUR 213

Caspase-4 Fluorometric Assay Kit

K126-400
EUR 925

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 468

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 615

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 229

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 958

Caspase-12 Fluorometric Assay Kit

K139-100
EUR 501

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 256

LDL Uptake Assay Kit (Cell-Based)

K436-100
EUR 680

Caspase 1 Colorimetric Cell-Based ELISA Kit (OKAG00577)

OKAG00577 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

Caspase 3 & 7 Assay Kit, green

KF17203 25 Tests
EUR 304

NucView 488 CASPASE 3 ASSAY KIT

30029 1KIT
EUR 429
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 Activity Assay Kit (Luminometric)

K186-100 Ask for price

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